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CO2 Incubator and In Vitro Fertilization The desire to have children is a fundamental hope for most couples in fulfilling their life’s plans but this can be very stressful for couples affected by fertility. IVF is an assisted reproductive technology procedure where fertilization occurs outside of the body in an incubator under controlled conditions. In Vitro Fertilization is used when female is the primary cause of the infertility (Endometriosis, Tubal disease, Hormonal dysregulation and Polycystic ovary syndrome) and Intracytoplasmic Sperm Injection (ICSI) is used in case of male infertility (if the quality of man sperms is inadequate). Incubators are vital to the overall success of IVF. They create the optimal, constant environment for embryo cultures. Good embryo culture conditions and subsequent embryonic development are dependent upon a correctly maintained incubator temperature and pH. The most commonly used environmental conditions for human IVF incubators are 5% CO2 in air, 37°C, and 100% relative humidity. CO2 Incubators are the most important equipment for the IVF because the eggs harvested from the ovaries of the patients for fertilization spend most of the time outside the body that is in the incubator. Because this unit gives the special environment conditions of the body like 37°C (body temperature) and 5-7 % CO2 maintain the physiological pH value of the cultural medium used and offer the best conditions for optimal oocyte and embryo development. CO2 is used to regulate the pH level, thus pH is measured and maintained by monitoring and adjusting the concentration of CO2. CO2 and pH have an inverse relationship as the concentration of CO2 increases, pH level decreases.


Gynaecology or gynecology[1] is the medical practice dealing with the health of the female reproductive system (uterus, vagina, and ovaries). Literally, outside medicine, it means "the science of women". Its counterpart is andrology, which deals with medical issues specific to the male reproductive system. Almost all modern gynaecologists are also obstetricians (see obstetrics and gynaecology). In many areas, the specialties of gynaecology and obstetrics overlap.


How we work

What is hysteroscopy?
Hysteroscopy provides a way for your physician to look inside your uterus. A hysteroscope is a thin, telescope-like instrument that is inserted into the uterus through the vagina and cervix. This tool often helps a physician diagnose or treat a uterine problem. Hysteroscopy is minor surgery which is performed either in your physician's office or in a hospital setting. It can be performed with local, regional, or general anesthesia--sometimes no anesthesia is needed. There is little risk involved with this procedure for most women.


What is laparoscopy?
Laparoscopy is a surgical procedure performed through very small incisions in the abdomen, using specialised instruments. The abdominal cavity is inflated with carbon dioxide gas (CO2) and distended. A pencil-thin instrument called a laparoscope is used; it has lenses like a telescope to magnify body structures, a powerful light to illuminate them, and a miniature video camera. The camera sends images of the inside of the body to a TV monitor in the operating room. Specialised surgical instruments can be inserted through the small incisions nearby. This type of surgery is called 'minimal access' because of the very small incisions used. Yet major procedures can now be performed using this technique.


IVF & ICSI :- Un-Assisted Reproduction
In order to understand assisted reproduction and how it can help infertile couples, it is important to understand how conception takes place naturally. In order for traditional conception to occur, the man must ejaculate his semen, the fluid containing the sperm, into the woman’s vagina near the time of ovulation, when her ovary releases an egg. Following ovulation, the egg is picked up by one of the fallopian tubes. Since fertilization usually takes place inside the fallopian tube, the man’s sperm must be capable of swimming through the vagina and cervical mucus, up the cervical canal into the uterus, and up into the fallopian tube, where it must attach to and penetrate the egg in order to fertilize it. The fertilized egg continues traveling to the uterus and implants in the uterine lining, where it grows and matures. If all goes well, a child is born approximately nine months later.

Dr. Kalyan B. Barmade Sir in IVF/ICSI Lab

Sperm bank , semen bank or cryobank
A sperm bank, semen bank or cryobank is a facility or enterprise that collects and stores human sperm from sperm donors for use by women who, for whatever reason, need donor-provided sperm to achieve pregnancy. Sperm donated by the sperm donor is known as donor sperm, and the process for introducing the sperm into the woman is called artificial insemination, which is a form of third party reproduction. From a medical perspective, a pregnancy achieved using donor sperm is no different from a pregnancy achieved using partner sperm, and it is also no different from a pregnancy achieved by sexual intercourse.


What is Egg Donation?
In egg donation, eggs are borrowed from a young woman (less than 33 yrs of age) called the donor, with her consent. These eggs are then fertilized with the sperms of the husband of the recipient woman and the resultant embryo (the earliest form of the baby), is inserted into the womb of the recipient. The success rate of this procedure is in the region of 40 to 50%. In fact, many women till the age of 50-55 have become pregnant by this technique. You will be surprised that the oldest woman pregnant by this procedure is 50 year old, with severe diabetes mellitus and severe hypertension.And she delivered a healthy female child by caesarean section At our Barmade Test Tube Baby Hospital. Barmade Hospital is an anonymous egg donation centre that provides personal attention and support to both egg donor and reciepient. Women after the age of 40 tend to have fewer eggs in their ovaries or the quality of the eggs they produce may be poor. Thus, even new technologies like IVF and ICSI may not ensure a successful pregnancy. Furthermore, after the age of 40 to 42, many women stop producing eggs as they enter the stage of Perimenopause (decreased periods) or menopause (stoppage of periods). Till now, such women could only have a baby through the wonderful route of adoption. However, in the last ten years, a new technique of egg donation has come as a blessing to many such women.

How to Work Egg Donation